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AvtorObradović, Marko
Strgulc-Krajšek, Simona
Dermastia, Marina
Kreft, Samo
Naslov A new method for the authentication of plant samples by analyzing fingerprint chromatograms / Marko Obradović ... [et al.]
Vrsta/vsebinatype of material članek - sestavni del
Fizični opisstr. 123-132
Predmetne oznake (nekontrolirane)capillary electrophoresis / chromatogram / plant sample authentication / multivariate analysis / Epilobium / Chamaenerion
PovzetekChemical analysis by high-performance liquid chromatography or capillary electrophoresis of plant pulverized samples, juices or extracts is an excellent method for the authentication of medicinal plant species and their products, particularly when morphological authentication is not possible. In the nventional procedure, chromatograms are integrated and the heights or areas of several peaks are used in a supervised pattern recognition method to confirm the authenticity of the product. We propose a new section approach in analysing chromatograms, where chromatograms are split into sections, which are described by four variables (number of peaks in the section, average retention time of peaks in the section, total area of peaks in the section andaverage area of peaks in the section), and these variables are then used instatistical analysis. The method is especially useful when the peaks on the chromatogram are not well separated and it is not easy to link individual peaks on one chromatogram with corresponding peaks on other chromatograms. In comparison with the standard procedure, our approach in analyzing chromatographic data of willow-herb (Epilobium and Chamaenerion spp.) extractswas more objective, gave better results and was also easier to perform.
Glej publikacijo TI=Phytochemical analysis ISSN: 0958-0344.- Vol. 18, no. 2 (2007), str. 123-132