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AvtorŠket, Primož, 1977-
Plavec, Janez
Naslov Tetramolecular DNA quadruplexes in solution : insights into structural diversity and cation movement / Primož Šket and Janez Plavec
Vrsta/vsebinatype of material članek - sestavni del
Jezikangleški
Leto2010
Fizični opisstr. 12724-12732
OpombeAbstract
Predmetne oznake (nekontrolirane)kemijska struktura snovi / oligonukleotidi / kvadrupleksi
UDK544.1
PovzetekThe present study expands the notion that the simple oligonucleotide sequence d(TG4T) in solution forms a tetramolecular G-quadruplex having a parallel orientation of the four strands and four G-quartets with all of the residues in the anti orientation. NMR experiments have revealed the equilibrium of two monomeric forms with a ratio between 85:15 and 70:30 in the presence of K+, Na+, and 15NH4+ ions. The major form consists of four G-quartets, whereas the minor form exhibits an additional T-quartet at the 5' end. An analogous oligonucleotide with U at the 5' end adopts a dimeric structure of G-quadruplex units in the presence of K+ and 15NH4+ cations but not in the presence of Na+ ions, where monomeric forms are present. Three 15NH4+ ion binding sites between the four G-quartets within the major monomeric form havebeen identified, while an additional 15NH4+ ion binding site between the G- and T-quartets at the 5' end of the minor form has been established. The dimeric d[(UG4T)4]2 G-quadruplex exhibits eight 15NH4+ ion binding sites, two of them between the U- and G-quartets. 15NH4+ ions have been shown to move faster between the interior of the tetramolecular structures and the bulk solution in comparison with the monomolecular and bimolecular G-quadruplexes. However, cation movement is slowed by the presence of a T-quartet at the 5' end.
COBISS.SI-ID4487962
Glej publikacijo TI=Journal of the American Chemical Society : JACS ISSN: 0002-7863.- Vol. 132, no. 36 (2010), str. 12724-12732