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AvtorCevec, Mirko
Thibaudeau, Christophe
Plavec, Janez
Naslov NMR structure of the let-7 miRNA interacting with the site LCS1 of lin-41 mRNA from Caenorhabditis elegans / Mirko Cevec, Christophe Thibaudeau and Janez Plavec
Vrsta/vsebinatype of material članek - sestavni del
Jezikangleški
Leto2010
Fizični opisstr. 7814-7821 : Ilustr.
OpombeBibliografija: str. 7821
Abstract
Predmetne oznake (nekontrolirane)kemija / kemijska struktura / jedrska magnetna resonanca / RNA
UDK54
Doi/ISAN10.1093/nar/gkq640
URLhttp://nar.oxfordjournals.org/content/38/21/7814.full?sid=cf397f6e-76ea-4b0a-818...
Povzetek We have determined the 3D structure of a 34-nt RNA construct, herein named LCS1co, which mimics the interaction of let-7 microRNA (miRNA) to one of its complementary binding sites, LCS1, in the 3'-untranslated region of lin-41 mRNA by solution-state NMR spectroscopy. let-7 miRNAs control the timing of development of the nematode Caenorhabditis elegans and are highly conserved in mammals. The sequence and structure of the two conserved let-7 complementary sites, LCS1 and LCS2, in the 3'-untranslated region of lin-41 mRNA are important for a proper downregulation of lin-41. The high-resolution NMR structure reveals details of the binding of let-7 miRNA to lin-41 mRNA which involves formation of a complex with non-canonical structural elements within the seed region. LCS1co exhibits a stem-loop structure with two stems, an asymmetric internal loop and an adenine bulge. Comparison with the NMR solution-state structure of the let-7:lin-41 complex involving the LCS2-binding site shows that conformational freedom of the asymmetric internal loop of LCS1co correlates with a smaller bend between the upper and lower stems in comparison to the well-defined asymmetric loop of LCS2co.
COBISS.SI-ID4554778
Glej publikacijo TI=Nucleic acids research ISSN: 0305-1048.- Vol. 38, no. 21 (2010), str. 7814-7821

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