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AuthorStres, Blaž
Tiedje, James M.
Murovec, Boštjan
Title BEsTRF : a tool for optimal resolution of terminal-restriction fragment length polymorphism (T-RFLP) analysis based on user defined primer-enzyme-sequence databases / Blaž Stres, James M. Tijede, Boštjan Murovec
Type/contenttype of material article - component part
LanguageEnglish
Publication date2009
Physical descriptionstr. 1556-1558
Uncontrolled subject headingsmikrobiologija / molekularna genetika / BEsTRF
UDC579:575
DOI/ISAN10.1093/bioinformatics/btp254
URLhttp://bioinformatics.oxfordjournals.org/cgi/reprint/btp254?ijkey=qw9y7y9LEcKhXd...
SummaryBEsTRF (Best Estimated T-RF) provides a stand-alone environment for analyzing primers-enzymes-gene section combinations used in T-RFLP for its optimal resolution. User defined sequence databases of several hundred thousand DNA sequences can be explored and the resolution of user specified sets of primers and restriction endonucleases can be analyzed on either forward or reverse terminal fragments. Sequence quality, primer mismatches, insertions and delitions can be controlled and each primer-pair specific sequence collections can be exported for downstream analyses. The configuration for a novel T-RFLP population profiling using rpoB gene (DNA-directed RNA polymerase, beta subunit) on forward fluorescently labelled primer are presented.
COBISS.SI-ID2450312
See publication: TI=ISMB/ECCB 2009 conference proceedings : June 27 to July 2, 2009, Stockholm, Sweden.- Issue 12, Vol. 25 (2009), str. 1556-1558

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